Summary
Type: SPE
Application #: 301730
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
1 column volume n-hexane, 1 column volume methanol, 1 column volume water (pH
2, acidified with HCl)
Sample application:
Slowly aspirate the sample through the column with vacuum; dry 15 min under
vacuum
Column washing:
Aspirate 1 ml n-hexane into the column packing, wait 2 min, aspirate solvent
through the column and wash with 1 ml n-hexane (chlorohydrocarbons); then dry
column under vacuum
Elution:
Aspirate 1 ml methanol into the column packing, wait 2 min, then wash with 1 ml
methanol
Authors: Macherey-Nagel
Source: LC Department